Identification of Alicyclobacillus sp. by molecular biology techniques

Identification of Alicyclobacillus sp. and guaiacol-producing Alicyclobacilli by PCR-RFLP and sequencing, using genus and species-specific primers. Strain identification will be carried out by standard PCR reactions (followed by RFLP and sequencing of the products) using primers designed for the housekeeping genes: rpoB and gyrB, as well as a fragment of the guaiacol synthesis involved vdc operon.

Microbiology 2 (Virology)

Virology, classification of viruses, their pathogenic potential and most important representatives. Viral biology and life cycle. Structure and properties. Viroids, prions, plasmids, and transposons. Defence mechanisms against intrusion of external genetic elements. Priciples of antiviral therapy and vaccination, antiviral agents. Mechanism of the tumorigenesis and on the oncogenic potential of various virus groups.

Microbiology 1

Microbial biology and biochemistry, including systematics, morphology, physiology, and diversity of biochemical processes of microorganisms, their ecological role, practical applications, introduction to methods of sterile laboratory work, bacterial isolation, quantification and identification.

Preservation and Control of Microbial Strains

Culture collections as Microbial Biological Resource Centres (mBRCs): role, functions, services and quality management systems. Main regulations affecting the use of microbial resources. Methods of preservation and recovery of eukaryotic and prokaryotic microorganisms (bacteria, fungi, and yeasts). Purity, viability, and authenticity testing, preparation of working strains and stock cultures and standardised inoculum. Classical microbiological techniques for phenotypic and physiological characterisation, molecular techniques based on nucleic acids, protein profiles, fatty acids, etc.

Isolation, cultivation and preservation of cyanobacterial strains

Hands-on training on field sampling, isolation, cultivation, and cryopreservation of cyanobacterial strains. Taxonomy of cyanobacteria. Morphological and molecular characterisation of cyanobacterial strains. Use of a Quality Management System to maintain a laboratory collection.