Catalogue of services
Based on its partner organisations’ state-of-the-art facilities/equipments and top-level expertise, MIRRI makes available for its users a vast, diverse portfolio of high-quality services. These range from general services to more application-specific ones – including pipelines of integrated, product-oriented services made available as tailor-made, turnkey solutions.
MIRRI’s services and expertise can help researchers and bioindustries delivering the maximum value and impacts from their projects, technologies and products.
An overview of the services made available by MIRRI is provided on the lists below.
Supply of biological material in different delivery forms (freeze-dried, active culture, cryovials, DNA, etc.)
Supply of freeze-dried biological material
Supply of actively growing cultures (agar plates or slopes or liquid media)
Supply of frozen material
Supply of genomic DNA
Other delivery forms for the supply of biological material (e.g., oil, water, etc.)
Characterisation based on the DNA/RNA sequence of molecular markers (housekeeping, rRNA, virulence, drug-resistance genes, etc.). Analysis of several markers (Multi-Locus Sequence Analysis/Typing, MLSA/MLST) allowing strain typing
DNA amplification by PCR of the gene(s) of interest, followed by sequencing of the amplified fragments
Strain typing based on differences in the DNA sequence of the isolates or environmental samples. Used, for example, for strain typing/differentiation, for phylogenetic analysis or to study the microbial diversity of environmental samples. The service includes consultancy to define the most suitable method considering the type of sample or the taxonomic group of the strain of interest
DNA amplification by PCR with a single oligonucleotide that hybridizes randomly to the DNA under study, which generates a unique fingerprint of each isolate
DNA amplification by PCR of a fragment from a region of interest (fixed size), followed by separation of the fragments in a gel electrophoresis with an increasing denaturing gradient. This method can detect single base changes/mutations in environmental samples
Digestion of the genomic DNA with specific restriction enzymes followed by amplification by PCR, which generates a unique fingerprint of each isolate
DNA amplification by PCR of microsatellite regions, which generates a unique fingerprint of each isolate
DNA amplification by PCR of extragenic repetitive palindromic elements, which generates a unique fingerprint of each isolate
DNA amplification by PCR of sequences separating Long Terminal Repeats retrotransposons, which generates a unique fingerprint of each isolate
Digestion of the genomic DNA with specific restriction enzymes, which generates a unique fingerprint of each isolate
Digestion of the mitochondrial DNA with specific restriction enzymes, which generates a unique fingerprint of each isolate
DNA amplification by PCR of the 16S rDNA gene, followed by digestion with specific restriction enzymes, which generates a unique fingerprint of each isolate. Can be used to make phylogenetic studies of environmental samples by cloning the 16S rDNA genes of the sample
Digestion of the genomic DNA with specific restriction enzymes and hybridization with a ribosomal (rRNA) probe, to analyse the number and sequence diversity of the rRNA operons
High-throughput dereplication screening using MALDI-TOF MS to group isolates that represent the same taxon. Used to reduce a large number of isolates to a smaller, non-redundant set for further characterization.
Comparison of protein mass spectra generated from different isolates to cluster them.
Separation of intact chromosomal DNAs by pulsed field gel electrophoresis (PFGE) to type at inter- and intra-specific level
Separation of intact chromosomal DNAs
Estimation of the number of copies of single chromosomes using flow cytometry and specific florescent dyes targeting DNA
Quantification of the DNA content in single cells and histogram analysis to estimate the level of ploidy
Characterisation of a strain based on presence, size and copy number of its plasmids. Used to type bacterial isolates
Determination of the presence, size and copy number of plasmids